DESCRIPTION Gene Name Synonym FNDC5, FRCP2, irisin, Irisin, fibronectin type III domain containing 5 Species Human A ccession Q8NAU1 Source HEK293 cells SPECIFICATIONS Amino acid sequence Asp32-Thr152 is expressed with a 10×His tag at the C terminus Molecular Weight 13.7 kDa Purity >95.0% as determined by SDS-PAGE Endotoxin <5 EU mg -1 Formulation Lyophilized from sterile PBS, pH 7.4.Normally 5%-8% trehalose and mannitol are added as protectants before lyophilization. Reconstitution Dissolved in sterile water, it is recommended to add 0.1% HSA or BSA for long-term preservation. STORAGE Stability and Storage 12 months from date of receipt at -20°C to -70°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for almost 6 months (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage. BACKGROUND Fibronectin type III domain-containing protein 5, the precursor of irisin, is a type I transmembrane glycoprotein that is encoded by the FNDC5 gene. Irisin is a cleaved version of FNDC5, named after the Greek messenger goddess Iris. Fibronectin domain-containing protein 5 is a membrane protein comprising a short cytoplasmic domain, a transmembrane segment, and an ectodomain consisting of a ~100 kDa fibronectin type III (FNIII) domain.Irisin, which was recently identified as a myokine and an adipokine, transforms white adipose tissue to brown adipose tissue.Irisin can potentially prevent obesity and associated type 2 diabetes by stimulating expression of WAT browning-specific genes via the p38 MAPK and ERK pathways.In addition to the proposed effects of irisin on adipose tissue, bone, and brain described in more detail below, irisin had anti-inflammatory and antimetastatic effects, improved glucose uptake in muscle cells, facilitated endothelial cell proliferation, lowered blood pressure, and improved cardiac hypertrophy. Data SDS-PAGE Bioactivity Recombinant Human FNDC5 Protein was determined by SDS-PAGE with Coomassie Blue. Active recombinant FNDC5 upregulated both UCP1 expression and white to brown fat conversion in mature 3T3-L1 adipocytes ( Immunofluorescence and Bodipy staining, Blue: DAPI; Green: Bodipy stain for Lipids; Red: Brown adipose tissue marker UCP1，cat: GB112174). （产品包装升级中，以实物为准。 ） 仅供科研用途，不可用于临床诊断！