产品信息 蛋白质全称 同源框蛋白SIX3 别名 HPE2, Sine oculis homeobox homolog 3, SIX homeobox 3 Uniprot ID Q62233 免疫原 KLH偶联的小鼠Six3合成肽 抗体亚型 IgG 预测分子量./观测分子量. 36 kDa / 37 kDa 纯化方式 亲和纯化 应用 应用 物种 稀释 阳性样品 WB 免疫印迹 人, 小鼠 1: 100-1: 300 C2C12细胞, SH-SY5Y细胞, 293T细胞 背景 Transcriptional regulator which can act as both a transcriptional repressor and activator by binding a ATTA homeodomain core recognition sequence on these target genes. During forebrain development represses WNT1 expression allowing zona limitans intrathalamica formation and thereby ensuring proper anterio-posterior patterning of the diencephalon and formation of the rostral diencephalon . Acts as a direct upstream activator of SHH expression in the rostral diencephalon ventral midline and that in turn SHH maintains its expression . In addition, Six3 activity is required for the formation of the telencephalon.Acts on the proliferation and differentiation of neural progenitor cells through activating transcription of CCND1 AND CCND2 . 图像 WB检测SIX3蛋白(货号 GB114815). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 300稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 5000稀释, 室温孵育1小时. 储存 储存条件 在-20℃下储存一年, 避免反复冻融. 储存缓冲液 含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液. 注意: 1.本产品仅供研究使用. 2.建议用一抗稀释液( G2025 )稀释本产品.