产品信息 蛋白质全称 PC4/SFRS1互作蛋白 别名 CLL associated antigen KW 7, DFS 70, DFS70, LEDGF, p52, p75, PAIP, PSIP1, PSIP2, Lens epithelium-derived growth factor, mLEDGF Uniprot ID Q99JF8 免疫原 小鼠PSIP1/LEDGF重组蛋白 抗体亚型 IgG 纯化方式 亲和纯化 亚细胞定位 细胞核 应用 应用 物种 稀释 阳性样品 IHC/IF 免疫组织化学/免疫荧光 小鼠, 大鼠 1: 1500-1: 3000 睾丸 背景 Transcriptional coactivator involved in neuroepithelial stem cell differentiation and neurogenesis. Involved in particular in lens epithelial cell gene regulation and stress responses. May play an important role in lens epithelial to fiber cell terminal differentiation. May play a protective role during stress-induced apoptosis. Isoform 2 is a more general and stronger transcriptional coactivator. Isoform 2 may also act as an adapter to coordinate pre-mRNA splicing. Cellular cofactor for lentiviral integration.. This protein has 2 isoforms produced by alternative splicing. 图像 IHC检测PSIP1/LEDGF蛋白(货号 GB113471). 样品: 小鼠睾丸, 4%多聚甲醛 (货号G1101) 固定12-24小时. 抗原修复: Tris-EDTA抗原修复液(pH 9.0) (G1203), 98℃, 20分钟. —抗: 1: 1500稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 200稀释, 室温孵育1小时. IHC检测PSIP1/LEDGF蛋白(货号 GB113471). 样品: 大鼠睾丸, 4%多聚甲醛 (货号G1101) 固定12-24小时. 抗原修复: Tris-EDTA抗原修复液(pH 9.0) (G1203), 98℃, 20分钟. —抗: 1: 1500稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 200稀释, 室温孵育1小时. 储存 储存条件 在-20℃下储存一年, 避免反复冻融. 储存缓冲液 含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液. 注意: 1.本产品仅供研究使用. 2.建议用一抗稀释液( G2025 )稀释本产品.