产品信息 蛋白质全称 核仁磷酸蛋白1 别名 140 kDa nucleolar phosphoprotein, Nopp140, Nolc1, Kiaa0035, NOPP130, NS5ATP13, Nucleolar 130 kDa protein, Nucleolar phosphoprotein p130 Uniprot ID E9Q5C9 免疫原 KLH偶联的小鼠NOLC1合成肽 抗体亚型 IgG 预测分子量./观测分子量. 74 kDa / 130 kDa 纯化方式 亲和纯化 应用 应用 物种 稀释 阳性样品 WB 免疫印迹 人 1: 500-1: 1000 K562细胞, A549细胞 背景 Nucleolar and coiled-body phosphoprotein 1 (NOLC1) is a phosphoprotein that transiently associates with the mature nucleolar H/ACA and C/D box small nucleolar ribonucleoproteins (snoRNPs), guiding site-specific 2'-O-meth-ylation and pseudouridylation of pre-rRNAs . It contains a nuclear localization signal binding sequence and is thought to shuttle between the nucleolus and the cytoplasm. NOLC1 plays an essential role in the synthesis of rRNA and the biosynthesis of ribosomes . 图像 WB检测NOLC1蛋白(货号 GB113095). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 1000稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 5000稀释, 室温孵育1小时. 储存 储存条件 在-20℃下储存一年, 避免反复冻融. 储存缓冲液 含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液. 注意: 1.本产品仅供研究使用. 2.建议用一抗稀释液( G2025 )稀释本产品.