产品信息 蛋白质全称 活化T细胞核因子胞质3 别名 NF-ATc3, NFATc3, NFATx, NFATC3, NFAT4, T-cell transcription factor NFAT4, NF-AT4, NF-AT4c Uniprot ID P97305 免疫原 小鼠NFATC3重组蛋白 抗体亚型 IgG 预测分子量./观测分子量. 116 kDa / 170 kDa 纯化方式 亲和纯化 应用 应用 物种 稀释 阳性样品 WB 免疫印迹 人 1: 500-1: 1000 293T细胞, Jurkat细胞, HeLa细胞 背景 NFATc4 (nuclear factor of activated T cells, cytoplasmic, calcineurindependent 4) is a member of the nuclear factors of activated T cells DNAbinding transcription complex that influences cytokine gene expression, cardiac hypertrophy and adipocyte differentiation. NFATc4 has specifically been implicated in vasculature development, regulation of smooth muscle contractile phenotype, and modulation of vascular smooth muscle contractility. NFATc4 plays a role in the inducible expression of cytokine genes in T cells, including the induction of IL-2 and IL-4. p38 MAP kinase phosphorylates multiple residues in the NFAT homology domain of NFATc4. 图像 WB检测NFAT4/NF-ATc3蛋白(货号 GB111453). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 500稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 5000稀释, 室温孵育1小时. 储存 储存条件 在-20℃下储存一年, 避免反复冻融. 储存缓冲液 含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液. 注意: 1.本产品仅供研究使用. 2.建议用一抗稀释液( G2025 )稀释本产品.