产品信息 蛋白质全称 SWI/SNF 相关、基质相关、肌动蛋白依赖性调节因子亚家族 E 成员 1 相关 别名 SMARCE1-related protein, BRCA2-associated factor 35, HMG box-containing protein 20B, Structural DNA-binding protein BRAF35, Hmg20b, Braf35, Hmgx2, Smarce1r Uniprot ID Q9Z104 免疫原 小鼠BRAF35重组蛋白 抗体亚型 IgG 预测分子量./观测分子量. 36 kDa / 36 kDa 纯化方式 亲和纯化 应用 应用 物种 稀释 阳性样品 WB 免疫印迹 人 1: 1000-1: 2000 293T细胞, A375细胞, Jurkat细胞 背景 CoREST (corepressor of repressor element-1-silencing transcription factor) is a chromatin- modifying complex that was first identified as a regulator of neuronal gene expression, having a essential role in neuronal differentiation . The CoREST complex is recruited by REST (RE1-silencing transcription factor) that recognizes the 21-nucleotide RE-1 sequence in the promoter of more than 1,700 different target genes in human and mouse . HMGB20B is one component of the CoREST complex, which mediates chromatin remodeling and repression of specific genes involved in neuronal differentiation through modulation of chromatin structure. 图像 WB检测BRAF35蛋白(货号 GB112980). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 2000稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 5000稀释, 室温孵育1小时. 储存 储存条件 在-20℃下储存一年, 避免反复冻融. 储存缓冲液 含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液. 注意: 1.本产品仅供研究使用. 2.建议用一抗稀释液( G2025 )稀释本产品.