产品信息 蛋白质全称 CREB调节转录共激活因子1 别名 Mucoepidermoid carcinoma translocated protein 1 homolog, TORC-1, Transducer of CREB protein 1, Crtc1, Kiaa0616, Mect1, Torc1, CRTC1, MECT1, TORC1, WAMTP1, CREB regulated transcription coactivator 1, MAML2, Mam-2 Uniprot ID Q68ED7 免疫原 KLH偶联的小鼠TORC1合成肽 抗体亚型 IgG 预测分子量./观测分子量. 67 kDa / 78 kDa 纯化方式 亲和纯化 应用 应用 物种 稀释 阳性样品 WB 免疫印迹 小鼠, 大鼠 1: 500-1: 1000 脑, 小脑, 海马区 背景 CRTC1, also named as MECT1, TORC1, and WAMTP1, belongs to the TORC family. It is a transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. CRTC1 acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated and acts independently of CREB1 'Ser-133' phosphorylation. It enhances the interaction of CREB1 with TAF4. CRTC1 regulates the expression of specific CREB-activated genes such as the steroidogenic gene, StAR. It is a potent coactivator of PGC1alpha and inducer of mitochondrial biogenesis in muscle cells. 图像 WB检测TORC1蛋白(货号 GB111000). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 500稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 5000稀释, 室温孵育1小时. 储存 储存条件 在-20℃下储存一年, 避免反复冻融. 储存缓冲液 含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液. 注意: 1.本产品仅供研究使用. 2.建议用一抗稀释液( G2025 )稀释本产品.