Product Information Description CREB mouse monoclonal Protein full name Cyclic AMP-responsive element-binding protein 1 Synonyms CREB1 , CREB, CREB-1, cAMP responsive element binding protein 1 Immunogen KLH conjugated Synthetic peptide corresponding to Human CREB-1. Isotype IgG Purity Affinity purification Predicted MW. 37 kDa Observed MW. 37 kDa Uniprot ID P16220 , Q01147 , P15337 Applications applications species dilution Positive tissue WB Human, Mouse, Rat 1: 500-1: 1000 spleen, lung, brain Background Cyclic AMP Response Element Binding protein (CREB) is a basic / leucine zipper transcription factor that binds the cyclic AMP response element (CRE) and activates transcription in response to a variety of extracellular signals including neurotransmitters, hormones, membrane depolarization, and growth and neurotrophic factors. Activation of CREB is dependent upon the phosphorylation of serine 133. Phosphorylation occurs via p44 / 42 MAP kinase and p90RSK and also via p38 MAP kinase and MSK 1. Although CREB will bind DNA independent of its phosphorylation state, only the phosphorylated form is competent as a transcription factor. CREB binding protein (CBP), a transcriptional coactivator that directly interacts with CREB, binds to CREB in the region of serine 133. CREB appears to play an important role in learning and memory. CREB knock out mice show diminished learning ability. Images Western blot analysis of CREB (GB13236-1) at dilution of 1: 1000. Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lane 3: NIH3T3 cell lysate Lane 4: Mouse spleen tissue lysate Lane 5: Mouse lung tissue lysate Lane 6: Mouse brain tissue lysate Lane 7: Rat spleen tissue lysate Lane 8: Rat lung tissue lysate Lane 9: Rat brain tissue lysate Storage Storage Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles. Storage Buffer PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. NOTE: 1.This product is intended for research only. 2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （ G2025 ）.