产品信息 蛋白质全称 固醇调节元件结合蛋白1 别名 bHLHd1, SREBF1, SREBP-1, SREBP 1c, SREBP1, Class D basic helix-loop-helix protein 1, Transcription factor SREBF1, SREBP1a, sterol regulatory element binding transcription factor 1 Uniprot ID Q9WTN3 免疫原 小鼠SREBP1重组蛋白 抗体亚型 IgG 预测分子量./观测分子量. 121 kDa / 54 kDa 纯化方式 亲和纯化 应用 应用 物种 稀释 阳性样品 WB 免疫印迹 人, 小鼠 1: 500-1: 1000 肺, 肾 背景 SREBP1, also known as SREBF1, a member of the basic helix-loop-helix-leucine zipper (bHLH-Zip) transcription factor family, are transcription factors that bind to a sequence in the promoter of different genes, called sterol regulatory element-1 (SRE1). The protein is synthesized as a precursor that is attached to the nuclear membrane and endoplasmic reticulum. Following cleavage, the mature protein translocates to the nucleus and activates transcription by binding to the SRE1. Sterols inhibit the cleavage of the precursor, and the mature nuclear form is rapidly catabolized, thereby reducing transcription. 图像 WB检测SREBP1蛋白(货号 GB11524). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 500稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 5000稀释, 室温孵育1小时. 储存 储存条件 在-20℃下储存一年, 避免反复冻融. 储存缓冲液 含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液. 注意: 1.本产品仅供研究使用. 2.建议用一抗稀释液( G2025 )稀释本产品.