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Recombinant Anti-Fascin antibody (Mouse mAb)

￥1650

货号：YB151036-100

品牌：YIKE

规格：

100 μL

数量：

Product Information

Protein full name	Fascin
Synonyms	Singed-like protein, Fscn1, Fan1, Snl, HSN, p55, SNL
Immunogen	KLH conjugated Synthetic peptide corresponding to Human Fascin
Uniprot ID	Q16658
Isotype	IgG1
Purity	Affinity purification
Subcellular location	Cytoplasm
Predicted MW. / Observed MW.	55 kDa / 55 kDa

Applications

Applications	Species	Dilution	Positive Tissue
WB	Human	1: 500-1: 1500	HeLa, Hep G2
IHC/IF	Human	1: 250-1: 1000	cervical carcinoma, liver cancer

Background

Fascin is a highly conserved and ubiquitous actin cross-linking protein that has a major function in cell motility and adhesion. Fascin localizes to a number of highly dynamic cellular structures that require strong mechanical support which include stress fibers and cellular protrusions such as microvilli, microspikes and lamellipodia. Fascin is up-regulated in many human carcinomas. It’s a marker for Reed-Sternberg cells in Hodgkin's disease; it is also a highly selective marker for dendritic cells of lymphoid tissues.

Images

	WB analysis of Fascin(GB151025). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 1500, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 3000, RT, 1h.
	IHC analysis of Fascin(GB151025). Sample: Human cervical carcinoma, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min. Primary antibody: 1: 500, 4℃ overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Mouse lgG(H+L), Ready to use(G1301), RT, 20min.
	IHC analysis of Fascin(GB151025). Sample: Human liver cancer, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min. Primary antibody: 1: 500, 4℃ overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Mouse lgG(H+L), Ready to use(G1301), RT, 20min.

Storage

Storage	Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer	PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.

NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.

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