Recombinant Anti - beta Actin antibody ( Rabbit mAb )
- 500 μg
Product Information
Protein full name | Actin, cytoplasmic 1 |
Synonyms | ACTB, BRWS1, PS1TP5BP1, Beta-actin, actin beta, Beta Cytoskeletal Actin, β-Actin |
Immunogen | KLH conjugated synthetic peptide corresponding to Mouse beta-Actin |
Uniprot ID | P60710 |
Isotype | IgG1 |
Purity | Affinity purification |
Subcellular location | Cytoplasm |
Predicted MW. / Observed MW. | 42 kDa / 45 kDa |
Formulation: | Lyophilized powder in PBS with 8% Mannitol, 0.01% Tween80. |
Size: | 500 μg |
Reconstitution: | Reconstitute with 2.5 mL sterile water first, and then add to 2.5 mL glycerin to final concentration of 0.1 mg/mL. |
Applications
WB | Human, Mouse, Rat | 0.02-0.1 μg/mL | HeLa, MCF-7, HepG2, Jurkat, PC-3, etc. |
IHC/IF | Human, Mouse, Rat | 0.1-0.2 μg/mL | spleen, colon cancer, lung, etc. |
ICC/IF | Human, Mouse, Rat | 0.1-0.2 μg/mL | NIH/3T3, A549, etc. |
Background
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility .
Images
| Western blot analysis of beta-Actin (GB15003) at dilution of 1: 2000 |
| Immunohistochemistry of paraffin embedded mouse spleen using beta-Actin (GB15003) at dilution of 1: 1000 |
| Immunohistochemistry of paraffin embedded human colon cancer using beta-Actin (GB15003) at dilution of 1: 1000 |
| Immunohistochemistry of paraffin embedded human lung cancer using beta-Actin (GB15003) at dilution of 1: 1000 |
| Immunohistochemistry of paraffin embedded rat lung using beta-Actin (GB15003) at dilution of 1: 1000 |
| Immunocytochemistry analysis of 4% paraformaldehyde fixed NIH/3T3 cells using β-Actin (GB15003) at dilution of 1: 1000 |
| Immunocytochemistry analysis of 4% paraformaldehyde fixed HeLa cells using β-Actin (GB15003) at dilution of 1: 1000 |
Storage
Storage | Avoid repeated freeze-thaw cycles. 6 months from date of receipt at 2-8°C; 12 months from date of receipt at -20°C; 24 months from date of receipt at -80°C; |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
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