Recombinant Anti-S6 Ribosomal Protein antibody (Mouse mAb)
- 100 μL
产品信息
蛋白质全称 | 小核糖体亚基蛋白质eS6 |
别名 | OK/SW-cl.2, RPS6, Phosphoprotein NP33, 40S ribosomal protein S6 |
Uniprot ID | P62753 |
免疫原 | 人 S6 Ribosomal Protein重组蛋白 |
抗体亚型 | IgG2a |
预测分子量./观测分子量. | 29 kDa / 32 kDa |
纯化方式 | 亲和纯化 |
应用
| 应用 | 物种 | 稀释 | 阳性样品 |
| WB 免疫印迹 | 人, 小鼠, 大鼠 | 1: 1000-1: 2000 | NIH3T3细胞, RAW264.7细胞, 肝 |
背景
Component of the 40S small ribosomal subunit .Plays an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA .Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome.
图像
| WB检测S6 Ribosomal Protein蛋白(货号 GB150087). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 1000稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗小鼠IgG (H+L) (货号GB23301), 1: 5000稀释, 室温孵育1小时. |
| WB检测S6 Ribosomal Protein蛋白(货号 GB150087). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 1000稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗小鼠IgG (H+L) (货号GB23301), 1: 5000稀释, 室温孵育1小时. |
储存
| 储存条件 | 在-20℃下储存一年, 避免反复冻融. |
| 储存缓冲液 | 含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
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