Recombinant Anti-HRP antibody (Mouse mAb)
- 100 μL
产品信息
蛋白质全称 | 辣根过氧化物酶 |
别名 | secondary, antibody, HRP, horse radish peroxidase, IgG, rabbit, ATTO, 15015 |
免疫原 | HRP重组蛋白 |
抗体亚型 | IgG1 |
克隆号 | SB593 |
纯化方式 | 亲和纯化 |
预测分子量. / 观测分子量. | 44 kDa / 44 kDa |
应用
| 应用 | 物种 | 稀释 | 阳性样品 |
| WB 免疫印迹 | Species independent | 1: 1000-1: 2000 | HRP 重组蛋白 |
背景
HRP (horseradish peroxidase) is an enzyme frequently used as an indicator in biochemical assays. It is used to amplify signals and enhance a target’s detectability. HRP acts on various chromogenic substrates to produce many different colors and acts on chemiluminescent substrates to produce light. HRP is often conjugated to an antibody to detect a target molecule. The antibody specifically locates the target, and HRP, in company with a substrate, and produces a detectable signal. Anti-HRP antibodies are designed to reliably detect HRP in various applications.
图像
| WB检测HRP蛋白(货号 YB150168). 样品: 经RIPA裂解液(货号Y2013)处理的蛋白质. 封闭: 3%脱脂牛奶(货号YC310012)溶于TBST溶液, 室温孵育1小时. —抗: 1: 1000稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗小鼠IgG (H+L) (货号YB23312), 1: 5000稀释, 室温孵育1小时. |
储存
| 储存条件 | 在-20℃下储存一年, 避免反复冻融. |
| 储存缓冲液 | 含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液. |
注意:
1.本产品仅供研究使用.
2.建议用一抗稀释液(Y2036)稀释本产品.
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