Recombinant Anti-RFP antibody (Rabbit mAb)
- 100 μL
产品信息
蛋白质全称 | 红色荧光蛋白drFP583 |
别名 | Red fluorescent protein, Red fluorescent protein eqFP611, RFPTag, RFP-Tag, RFP Tag |
Uniprot ID | Q9U6Y8 |
免疫原 | RFP重组蛋白 |
抗体亚型 | IgG, κ |
克隆号 | SB299 |
纯化方式 | 亲和纯化 |
应用
| 应用 | 物种 | 稀释 | 阳性样品 |
| ICC/IF 免疫细胞化学/免疫荧光 | Species independent | 1: 500-1: 2000 | expi293细胞 |
背景
Fluorescent proteins have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions.Red Fluorescent Protein (RFP) is a versatile biological marker for monitoring physiological processes, visualizing protein localization, and detecting transgenic expression in vivo. RFP can be excited by the 488 nm or 532 nm laser line and is optimally detected at 588 nm.
图像
| ICC检测RFP蛋白(货号 YB150046)(红色）. 样品: 293细胞, 4%多聚甲醛 (货号Y1112) 固定20分钟. 封闭: 3% BSA（货号YC305021）的PBS溶液, 室温孵育30分钟. —抗: 1: 1000稀释, 4℃ 孵育过夜. 二抗: Cy3标记山羊抗兔IgG (H+L) (货号YB21314), 1: 300稀释, 室温孵育1小时. |
储存
| 储存条件 | 在-20℃下储存一年, 避免反复冻融. |
| 储存缓冲液 | 含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液. |
注意:
1.本产品仅供研究使用.
2.建议用一抗稀释液(Y2036)稀释本产品.
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