产品信息

蛋白质全称	推定丝氨酸蛋白酶PRSS50
别名	Cancer/testis antigen 20, CT20, PRSS50, Serine protease 50, Testis-specific protease-like protein 50, TSP50
Uniprot ID	Q8BLH5
免疫原	小鼠TSP50重组蛋白
抗体亚型	IgG
预测分子量./观测分子量.	49 kDa / 45 kDa
纯化方式	亲和纯化

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应用

应用	物种	稀释	阳性样品
WB 免疫印迹	小鼠, 大鼠	1: 2000-1: 4000	脑, 肾, 睾丸

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背景

TSP50 (Testis-specific protease-like protein 50) is also named as PRSS50, CT20 and belongs to the peptidase S1 family. It can enhance the expression of NFKB target genes involved in cell proliferation, and promotes cell proliferation, at least partially, through activation of the NFKB signalling pathway. There are two protein bands of 37 kDa and 43 kDa to be detected in human testes that the the difference is most likely caused by posttranslational proteolytic cleavage. The full length protein has a signal peptide with 39 amino acids and two glycosylation sites.

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图像

	WB检测TSP50蛋白(货号 GB113646). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 4000稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 5000稀释, 室温孵育1小时.
	WB检测TSP50蛋白(货号 GB113646). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 4000稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 5000稀释, 室温孵育1小时.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(G2025)稀释本产品.