产品信息

蛋白质全称	Ras GTP酶激活样蛋白IQGAP3
别名	IQGAP3, IQ motif containing GTPase activating protein 3, MGC1947
Uniprot ID	Q86VI3
免疫原	人IQGAP3重组蛋白
抗体亚型	IgG
纯化方式	亲和纯化
亚细胞定位	细胞膜, 细胞质

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应用

应用	物种	稀释	阳性样品
IHC/IF 免疫组织化学/免疫荧光	人	1: 700-1: 1400	胎盘

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背景

IQGAP3 (IQ motif containing GTPase activating protein 3) is a 1,631 amino acid protein that acts as an effector of Cdc42 and Rac 1, linking their activation to the cytoskeleton during neuronal morphogenesis. A novel member of the IQGAP family, IQGAP3 is highly expressed in brain where it localizes to axons of hippocampal neurons. IQGAP3 contains one Ras-GAP domain, a CH (calponin-homology) domain, four IQ domains and is encoded by a gene located on human chromosome 1, which spans 260 million base pairs, contains over 3,000 genes and comprises nearly 8% of the human genome.

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图像

IHC检测IQGAP3蛋白(货号 GB111751). 样品: 人胎盘, 4%多聚甲醛 (货号G1101) 固定12-24小时. 抗原修复: 柠檬酸抗原修复液(干粉, pH 6.0) (G1201), 98℃, 20分钟. —抗: 1: 700稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 200稀释, 室温孵育1小时.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(G2025)稀释本产品.