产品信息

蛋白质全称	衔接分子Crk
别名	CRK, Crk p38, CRK isoform 2, CrkII; CRKII; p38, Proto oncogene C crk, v crk sarcoma virus CT10 oncogene homolog, Adapter molecule crk, Avian sarcoma virus CT10
Uniprot ID	P46108
免疫原	人Adapter molecule crk重组蛋白
抗体亚型	IgG
预测分子量./观测分子量.	34 kDa / 38 kDa
纯化方式	亲和纯化

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应用

应用	物种	稀释	阳性样品
WB 免疫印迹	人	1: 500-1: 1000	HepG2细胞, A549细胞, MCF7细胞

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背景

Adapter molecule crk also known as proto-oncogene c-Crk or p38 is a member of an adapter protein family that binds to several tyrosine-phosphorylated proteins. This protein has several SH2 and SH3 domains (src-homology domains) and is involved in several signaling pathways, recruiting cytoplasmic proteins in the vicinity of tyrosine kinase through SH2-phosphotyrosine interaction. The N-terminal SH2 domain of this protein functions as a positive regulator of transformation whereas the C-terminal SH3 domain functions as a negative regulator of transformation.

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图像

WB检测Crk p38蛋白(货号 GB11645). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 500稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 5000稀释, 室温孵育1小时.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(G2025)稀释本产品.