产品信息

蛋白质全称	β-2-微球蛋白
别名	Beta-2-microglobulin form pI 5.3, B2M, CDABP0092, HDCMA22P, B2m, Hdcma22p, Beta chain of mhc class 1 proteins, IMD43
Uniprot ID	P01887
免疫原	小鼠beta 2 Microglobulin重组蛋白
抗体亚型	IgG
预测分子量./观测分子量.	14 kDa / 14 kDa
纯化方式	亲和纯化

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应用

应用	物种	稀释	阳性样品
WB 免疫印迹	人	1: 500-1: 1000	Jurkat细胞, HepG2细胞, Raji细胞

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背景

This gene encodes a serum protein found in association with the major histocompatibility complex (MHC) class I heavy chain on the surface of nearly all nucleated cells. Involved in the presentation of peptide antigens to the immune system. Exogenously applied M.tuberculosis EsxA or EsxA-EsxB (or EsxA expressed in host) binds B2M and decreases its export to the cell surface (total protein levels do not change), probably leading to defects in class I antigen presentation.

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图像

WB检测beta 2 Microglobulin蛋白(货号 GB111119). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 500稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 5000稀释, 室温孵育1小时.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(G2025)稀释本产品.