产品信息

蛋白质全称	胱抑素C
别名	Cystatin-3, Cst3, AD8, ARMD11, CYTC, Epididymis secretory protein Li 2, Gamma trace, HCCAA, MGC117328, Neuroendocrine basic polypeptide, Post gamma globulin
Uniprot ID	P21460
免疫原	小鼠Cystatin C重组蛋白
抗体亚型	IgG
预测分子量./观测分子量.	16 kDa / 14 kDa
纯化方式	亲和纯化

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应用

应用	物种	稀释	阳性样品
WB 免疫印迹	小鼠	1: 500-1: 1000	睾丸, 脾, 脑

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背景

Cystatin C is a inhibitor of cysteine proteinases which is secreted by all cell types and is completely cleared from the organism through glomerular filtration, shown to be an early and sensitive biomarker of renal dysfunction. It is also used as an emerging biomarker in cardiovascular disease. Cystatin C is involved in a variety of inflammatory reactions. The concentration of serum cystatin C has also been shown to be unaltered in certain inflammatory conditions or other disorders of metabolism.

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图像

WB检测Cystatin C蛋白(货号 GB111376). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 500稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 5000稀释, 室温孵育1小时.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(G2025)稀释本产品.