Recombinant Anti-TSG101 antibody (HRP Conjugated)
- 100 μL
产品信息
蛋白质全称 | 肿瘤易感基因101蛋白 |
别名 | TSG10, TSG101, tumor susceptibility gene 101, VPS23, tumor susceptibility 101, Cell and organelle markers, ESCRT I complex subunit TSG101, Exosome marker |
Uniprot ID | Q61187 |
免疫原 | KLH偶联的人TSG101合成肽 |
预测分子量./观测分子量. | 44 kDa / 46 kDa |
纯化方式 | 亲和纯化 |
偶联 | 辣根过氧化物酶 |
应用
| 应用 | 物种 | 稀释 | 阳性样品 |
| WB 免疫印迹 | 小鼠, 大鼠 | 1: 500-1: 1000 | 心, 脑 |
| 孵化条件 | 封闭后, 将膜与HRP标记的抗TSG101 抗体(ZB150022-HRP)在室温（20-25℃）孵育3-4小时或4℃过夜, 随后用TBST缓冲液洗涤3次, 每次5分钟. | ||
背景
Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Binds to ubiquitinated cargo proteins and is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs). Mediates the association between the ESCRT-0 and ESCRT-I complex. Required for completion of cytokinesis; the function requires CEP55. May be involved in cell growth and differentiation. Acts as a negative growth regulator. Required for the exosomal release of SDCBP, CD63 and syndecan (By similarity).
图像
| WB检测TSG101蛋白(货号 ZB150022-HRP). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 3% BSA（货号GC305010）的PBS溶液, 室温孵育30分钟. 抗体: HRP标记的抗TSG101蛋白抗体 1: 500稀释, 4℃ 孵育过夜. |
储存
| 储存条件 | 在-20℃下储存一年, 避免反复冻融. |
| 储存缓冲液 | 含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.产品颜色为浅黄色. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
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