产品信息

蛋白质全称	mCherry荧光蛋白
别名	Monomeric cherry red fluorescent protein
免疫原	mCherry重组蛋白
纯化方式	亲和纯化
偶联	辣根过氧化物酶

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应用

应用	物种	稀释	阳性样品
WB 免疫印迹	Species independent	1: 500-1: 2000	mCherry 重组蛋白
孵化条件	封闭后, 将膜与HRP标记的抗mCherry 抗体 (ZB152058-HRP)在室温（20-25℃）孵育3-4小时或4℃过夜, 随后用TBST缓冲液洗涤3次, 每次5分钟.

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背景

mCherry is derived from proteins originally isolated from Cnidarians (jelly fish, sea anemones and corals), and is used as a fluorescent tracer in trasfection and transgenic experiments. The prototype for these fluorescent proteins is Green Fluorescent Protein (GFP), which is a ~27kDa protein isolated originally from the jellyfish Aequoria victoria. The mCherry protein is derived from DsRed, a red fluorescent protein related to GFP isolated from so-called disc corals of the genus Discosoma.

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图像

WB检测mCherry(货号 ZB152058-HRP). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. 抗体: HRP标记的抗mCherry 抗体 1: 500稀释, 4℃ 孵育过夜.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.产品颜色为浅黄色.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(G2025)稀释本产品.