Recombinant Anti-Hsp70 antibody (HRP Conjugated)
- 50 μL
产品信息
蛋白质全称 | 热休克70kDa蛋白1A |
别名 | HSP70-1, HSP70.1, HSPA1, HSP72, Heat Shock 70 KDa Protein 1 |
Uniprot ID | P0DMV8 |
免疫原 | 人HSP70重组蛋白 |
预测分子量./观测分子量. | 70 kDa / 70 kDa |
纯化方式 | 亲和纯化 |
偶联 | 辣根过氧化物酶 |
应用
| 应用 | 物种 | 稀释 | 阳性样品 |
| WB 免疫印迹 | 人, 小鼠, 大鼠 | 1: 500-1: 2000 | 脑, HeLa细胞, Hep G2细胞 |
| 孵化条件 | 封闭后, 将膜与HRP标记的抗HSP70 抗体(ZB15241-HRP)在室温（20-25℃）孵育3-4小时或4℃过夜, 随后用TBST缓冲液洗涤3次, 每次5分钟. | ||
背景
This protein is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins.
图像
| WB检测HSP70蛋白(货号 ZB15241-HRP). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. 抗体: HRP标记的抗HSP70蛋白 抗体 1: 2000稀释, 4℃ 孵育过夜. |
储存
| 储存条件 | 在-20℃下储存一年, 避免反复冻融. |
| 储存缓冲液 | 含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.产品颜色为浅黄色. |
注意:
1.本产品仅供研究使用.
2.建议用一抗稀释液(G2025)稀释本产品.
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