Recombinant Anti-beta Catenin antibody (HRP Conjugated)
- 100 μL
产品信息
蛋白质全称 | 连环蛋白β1 |
别名 | Beta catenin, beta-Catenin, Catenin beta 1, CTNNB, CTNNB1, PRO2286, β catenin, β-catenin, Cadherin associated protein, armadillo, catenin beta 1, CATNB |
Uniprot ID | P35222 |
免疫原 | 重组人beta catenin protein在E.coli中表达 |
预测分子量./观测分子量. | 85 kDa / 92 kDa |
纯化方式 | 亲和纯化 |
偶联 | 辣根过氧化物酶 |
应用
| 应用 | 物种 | 稀释 | 阳性样品 |
| WB 免疫印迹 | 人, 小鼠, 大鼠 | 1: 500-1: 1000 | A-431细胞, 脑 |
| 孵化条件 | 封闭后, 将膜与HRP标记的抗beta Catenin 抗体(ZB150016-HRP)在室温（20-25℃）孵育3-4小时或4℃过夜, 随后用TBST缓冲液洗涤3次, 每次5分钟. | ||
背景
β-catenin is a dual function protein, involved in regulation and coordination of cell–cell adhesion and gene transcription. In humans, the CTNNB1 protein is encoded by the CTNNB1 gene. In Drosophila, the homologous protein is called armadillo. β-catenin is a subunit of the cadherin protein complex and acts as an intracellular signal transducer in the Wnt signaling pathway. It is a member of the catenin protein family and homologous to γ-catenin, also known as plakoglobin.
图像
| WB检测beta Catenin蛋白(货号 ZB150016-HRP). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 3% BSA（货号GC305010）的PBS溶液, 室温孵育30分钟. 抗体: HRP标记的抗beta Catenin蛋白抗体 1: 500稀释, 4℃ 孵育过夜. |
储存
| 储存条件 | 在-20℃下储存一年, 避免反复冻融. |
| 储存缓冲液 | 含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.产品颜色为浅黄色. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
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