Anti-TLR4 antibody (HRP Conjugated)
- 50 μL
产品信息
蛋白质全称 | Toll样受体4 |
别名 | Lps,TLR4, ARMD10, CD284, TLR-4, TOLL, toll like receptor 4 |
Uniprot ID | Q9QUK6 |
免疫原 | KLH偶联的小鼠TLR4合成肽 |
预测分子量./观测分子量. | 95 kDa / 95 kDa |
纯化方式 | 亲和纯化 |
偶联 | 辣根过氧化物酶 |
应用
| 应用 | 物种 | 稀释 | 阳性样品 |
| WB 免疫印迹 | 人, 小鼠, 大鼠 | 1: 500-1: 1000 | THP-1细胞, RAW264.7细胞, 胸腺 |
| 孵化条件 | 封闭后, 将膜与HRP标记的抗TLR4 抗体(ZB11519-HRP)在室温（20-25℃）孵育3-4小时或4℃过夜, 随后用TBST缓冲液洗涤3次, 每次5分钟. | ||
背景
TLR4 is a transmembrane protein, member of the toll-like receptor family, which belongs to the pattern recognition receptor (PRR) family. Its activation leads to an intracellular signaling pathway NF-κB and inflammatory cytokine production which is responsible for activating the innate immune system.It is most well known for recognizinglipopolysaccharide (LPS), a component present in many Gram-negative bacteria (e.g. Neisseria spp.) and selectGram-positive bacteria. Its ligands also include several viral proteins, polysaccharide, and a variety of endogenous proteins such as low-density lipoprotein, beta-defensins, and heat shock protein.
图像
| WB检测TLR4蛋白(货号 ZB11519-HRP). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 3% BSA（货号GC305010）的PBS溶液, 室温孵育30分钟. 抗体: HRP标记的抗TLR4蛋白抗体 1: 500稀释, 4℃ 孵育过夜. |
| WB检测TLR4蛋白(货号 ZB11519-HRP). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 3% BSA（货号GC305010）的PBS溶液, 室温孵育30分钟. 抗体: HRP标记的抗TLR4蛋白抗体 1: 500稀释, 4℃ 孵育过夜. |
储存
| 储存条件 | 在-20℃下储存一年, 避免反复冻融. |
| 储存缓冲液 | 含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.产品颜色为浅黄色. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
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