产品信息

蛋白质全称	增强型绿色荧光蛋白
别名	Enhanced Green Fluorecent protein, rEGFP, Recombinant EGFP, Enhanced Green Fluorecent protein, eGFP, EGFP
Uniprot ID	P42212
免疫原	EGFP( enhanced green fluorescent protein)重组蛋白
纯化方式	亲和纯化
偶联	辣根过氧化物酶

---

应用

应用	物种	稀释	阳性样品
WB 免疫印迹	Species independent	1: 1000-1: 2000	eGFP融合蛋白
孵化条件	封闭后, 将膜与HRP标记的抗eGFP 抗体(ZB15603-HRP)在室温（20-25℃）孵育3-4小时或4℃过夜, 随后用TBST缓冲液洗涤3次, 每次5分钟.

---

背景

Enhanced green fluorescence protein (eGFP) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelenth of 509 nm) when excited by blue light (excitation peak at a wavelenth of 395 nm). eGFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. eGFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize eGFP-tagged proteins within living cells without the need for chemical staining.

---

图像

WB检测EGFP蛋白(货号 ZB15603-HRP). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. 抗体: HRP标记的抗EGFP蛋白 抗体 1: 2000稀释, 4℃ 孵育过夜.

---

储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.产品颜色为浅黄色.

---

注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(G2025)稀释本产品.