产品信息

蛋白质全称	His标签
别名	6*His, His Tag, 6histag, 6XHis, HHHHHH, HIS, His probe, His Tag, His-probe, HISTIDINE TAGGED
免疫原	KLH偶联的HHHHHH (6* His)合成肽
纯化方式	亲和纯化
偶联	辣根过氧化物酶

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应用

应用	物种	稀释	阳性样品
WB 免疫印迹	Species independent	1: 2000-1: 5000	His-Tag融合蛋白
孵化条件	封闭后, 将膜与HRP标记的抗His Tag 抗体(ZB151255-HRP)在室温（20-25℃）孵育3-4小时或4℃过夜, 随后用TBST缓冲液洗涤3次, 每次5分钟.

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背景

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. His-tag is often used for affinity purification and binding assays. Expressed His-tagged proteins can be purified and detected easily because the string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper, under specific buffer conditions. The His-tag antibody is a useful tool for monitoring of the His-tagged proteins, and recognizes His-tags placed at N-terminal, C-terminal, and internal regions of fusion proteins expressed in bacteria, insect, and mammalian cells.

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图像

WB检测His Tag(货号 ZB151255-HRP). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. 抗体: HRP标记的抗His Tag 抗体 1: 5000稀释, 4℃ 孵育过夜.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.产品颜色为浅黄色.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(G2025)稀释本产品.