产品信息

蛋白质全称	胶质纤维酸性蛋白
别名	GFAP, ALXDRD, glial fibrillary acidic protein, Astrocyte, Intermediate filament protein
Uniprot ID	P03995
免疫原	KLH偶联的小鼠GFAP合成肽
预测分子量./观测分子量.	49 kDa / 49 kDa
纯化方式	亲和纯化
偶联	辣根过氧化物酶

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应用

应用	物种	稀释	阳性样品
WB 免疫印迹	小鼠, 大鼠	1: 1000-1: 2000	脑, 大脑皮层, 脊髓

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背景

Glial fibrillary acidic protein is an intermediate filament (IF) protein that is expressed by numerous cell types of the central nervous system (CNS) including astrocytes and ependymal cells.GFAP has also been found to be expressed in glomeruli and peritubular fibroblasts taken from rat kidneys Leydig cells of the testis in both hamsters and humans, human keratinocytes, human osteocytes and chondrocytes and stellate cells of the pancreas and liver in rats. It is closely related to its non-epithelial family members, vimentin,desmin, and peripherin, which are all involved in the structure and function of the cell’s cytoskeleton.

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图像

WB检测GFAP蛋白(货号 ZB12096-HRP). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. 抗体: HRP标记的抗GFAP蛋白 抗体 1: 1000稀释, 4℃ 孵育过夜.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.产品颜色为浅黄色.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(G2025)稀释本产品.