产品信息

蛋白质全称	BPI折叠家族B成员2
别名	PIL1, C20orf184, LPLUNC2, RYSR
Uniprot ID	Q8C1E1
免疫原	KLH偶联的小鼠BPIL1合成肽
抗体亚型	IgG
预测分子量./观测分子量.	50 kDa / 50 kDa
纯化方式	亲和纯化

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应用

应用	物种	稀释	阳性样品
WB 免疫印迹	人	1: 2000-1: 3000	Hep G2细胞, K562细胞

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背景

Members of the bactericidal/permeability-increasing protein family have antimicrobial properties and bind lipophilic substances, therefore targeting gram-negative bacteria. The bactericidal permeability increasing protein (BPI) is an antibacterial and endotoxin-neutralizing molecule that is abundant in the granules of polymorphonuclear leukocytes (neutrophil granules). Sharing structural and sequence homologies with BPI, BPIL1 (bactericidal/permeability-increasing protein-like 1) is a 458 amino acid secreted protein that contains the family’s common conserved feature of two cysteine residues that are critical for protein function.

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图像

WB检测BPIL1蛋白(货号 GB115240). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 2000稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 5000稀释, 室温孵育1小时.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(G2025)稀释本产品.