产品信息

蛋白质全称	法尼基焦磷酸合酶
别名	FDPS, FPP synthase, FPPS, FPS, KIAA1293, Cholesterol-regulated 39 kDa protein, CR 39, Dimethylallyltranstransferase, Farnesyl diphosphate synthase, Geranyltranstransferase
Uniprot ID	Q920E5
免疫原	小鼠FDPS/FPS重组蛋白
抗体亚型	IgG
预测分子量./观测分子量.	41 kDa / 39 kDa
纯化方式	亲和纯化

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应用

应用	物种	稀释	阳性样品
WB 免疫印迹	人	1: 500-1: 1000	HeLa细胞, HUVEC细胞, HCT116细胞

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背景

FPPS (also known as FDPS) is a key enzyme in isoprenoid biosynthesis which catalyzes the formation of farnesyl diphosphate (FPP), a precursor for several classes of essential metabolites including sterols, dolichols, carotenoids, and ubiquinones. The expression of FPPS was elevated in prostate tumor cell lines, indicating its potential role in prostate cancer progression. Western blot analysis using this antibody detected a major band around 41 kDa in mouse liver tissue.

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图像

WB检测FDPS/FPS蛋白(货号 GB113628). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 1000稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 5000稀释, 室温孵育1小时.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(G2025)稀释本产品.