产品信息

蛋白质全称	二酰甘油脂肪酶β
别名	DAGL-beta, DAGLbeta, DGL-beta, PUFA-specific triacylglycerol lipase, Sn1-specific diacylglycerol lipase beta, Daglb, KCCR13L
Uniprot ID	Q91WC9
免疫原	小鼠DAGLB重组蛋白
抗体亚型	IgG
预测分子量./观测分子量.	74 kDa / 74 kDa
纯化方式	亲和纯化

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应用

应用	物种	稀释	阳性样品
WB 免疫印迹	人, 小鼠, 大鼠	1: 500-1: 1000	脑, 睾丸

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背景

DAGL beta uses calcium as a cofactor to catalyze the hydrolysis of diacylglycerol (DAG) to 2-arachidonoyl-glycerol (2-AG), a reaction that is required for axonal growth and for retrograde synaptic signaling at mature synapses. DAGL beta functions at an optimal pH of 7 and its activity is inhibited by p-hydroxy-mercuri-benzoate and HgCl2, but not PMSF. There are three isoforms of DAGL beta that are produced as a result of alternative splicing events.

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图像

WB检测DAGLB蛋白(货号 GB113076). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 1000稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 5000稀释, 室温孵育1小时.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(G2025)稀释本产品.