产品信息

蛋白质全称	乙酰辅酶A硫酯酶
别名	Acyl-CoA thioester hydrolase 12, STARD15, Acyl-coenzyme A thioesterase 12, ACOT12, Acyl-CoA thioesterase 12, Cytoplasmic acetyl-CoA hydrolase 1, CACH-1, CACH1, hCACH-1, START domain-containing protein 15, CACH
Uniprot ID	Q8WYK0
免疫原	KLH偶联的人ACOT12合成肽
抗体亚型	IgG
预测分子量./观测分子量.	62 kDa / 62 kDa
纯化方式	亲和纯化

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应用

应用	物种	稀释	阳性样品
WB 免疫印迹	人, 小鼠, 大鼠	1: 500-1: 1000	肝, 胰腺

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背景

Acyl-coenzyme A thioesterase 12/ACOT12 is a member of the Acyl-CoA thioesterases family .Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. Acyl-coenzyme A thioesterase 12/ACOT12 preferentially hydrolyzes acetyl-CoA.

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图像

	WB检测ACOT12蛋白(货号 GB111565). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 500稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 5000稀释, 室温孵育1小时.
	WB检测ACOT12蛋白(货号 GB111565). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 500稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 5000稀释, 室温孵育1小时.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(G2025)稀释本产品.