产品信息

蛋白质全称	磷脂酰肌醇4-磷酸5-激酶1α
别名	PIP5K1-alpha, PtdIns(4)P-5-kinase 1 alpha, Pip5k1a, 68 kDa type I phosphatidylinositol 4-phosphate 5-kinase, Phosphatidylinositol 4-phosphate 5-kinase type I alpha, Pip5k1b, PIP5KIalpha, Phosphatidylinositol 4-phosphate 5-kinase type I beta
Uniprot ID	P70182
免疫原	小鼠PIP5K1A重组蛋白
抗体亚型	IgG
预测分子量./观测分子量.	60 kDa / 68 kDa
纯化方式	亲和纯化

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应用

应用	物种	稀释	阳性样品
WB 免疫印迹	人, 小鼠, 大鼠	1: 500-1: 1000	脑, 肺, 肝

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背景

Phosphatidylinositol 4-phosphate 5-kinases (PIP5Ks) play diverse roles in the cellular biology of many organisms, including signal transduction, secretion and vesicular trafficking, and regulation of cytoskeleton assembly. There are three PIP5K isoforms,αandβ, and γ. The nomenclature for the αandβ isoforms is switched between humans and mice. There are also several splicing variants of the g isozyme have been identified. This protein has 4 isoforms produced by alternative splicing.

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图像

WB检测PIP5K1 alpha/PIP5K1A蛋白(货号 GB111059). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 500稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 5000稀释, 室温孵育1小时.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(G2025)稀释本产品.