产品信息

蛋白质全称	胆盐激活脂肪酶
别名	BAL, Bile salt stimulated lipase, Bile salt-activated lipase, BSDL, BSSL, Bucelipase, Carboxyl ester lipase, CEase, CEL, CELL, Cholesterol esterase, FAP, FAPP, LIPA, MODY8, Pancreatic lysophospholipase, Sterol esterase
Uniprot ID	P19835
免疫原	KLH偶联的人Bile salt-activated lipase合成肽
抗体亚型	IgG
预测分子量./观测分子量.	79 kDa / 70 kDa
纯化方式	亲和纯化

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应用

应用	物种	稀释	阳性样品
WB 免疫印迹	人	1: 1000-1: 2000	THP-1细胞

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背景

Catalyzes the hydrolysis of a wide range of substrates including cholesteryl esters, phospholipids, lysophospholipids, di- and tri-acylglycerols, and fatty acid esters of hydroxy fatty acids (FAHFAs). Preferentially hydrolyzes FAHFAs with the ester bond further away from the carboxylate. Unsaturated FAHFAs are hydrolyzed more quickly than saturated FAHFAs (By similarity). Has an essential role in the complete digestion of dietary lipids and their intestinal absorption, along with the absorption of fat-soluble vitamins.

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图像

WB检测Bile salt-activated lipase蛋白(货号 GB115262). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 1000稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 5000稀释, 室温孵育1小时.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(G2025)稀释本产品.