产品信息

蛋白质全称	血管紧张素原
别名	AGT, ANHU, SERPINA8, angiotensinogen, hFLT1, Ang I, Ang II, Ang III, Angiotensin I, Angiotensin II, Angiotensin III, Angiotensin-3 , ANRT, AT-2, PAT , SERPINA8, Alpha 1 antiproteinase antitrypsin
Uniprot ID	P11859
免疫原	小鼠Angiotensinogen重组蛋白
抗体亚型	IgG
预测分子量./观测分子量.	52 kDa / 52 kDa
纯化方式	亲和纯化

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应用

应用	物种	稀释	阳性样品
WB 免疫印迹	小鼠	1: 500-1: 1000	肝, 肾

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背景

Angiotensinogen is an α-2-globulin produced constitutively and released into the circulation mainly by the liver, It is a member of the serpin family.Angiotensin is a peptide hormone that causes vasoconstriction and a subsequent increase in blood pressure. It is part of the renin-angiotensin system, which is a major target for drugs that raises blood pressure. Angiotensin also stimulates the release of aldosterone, another hormone, from the adrenal cortex. Aldosterone promotes sodium retention in the distal nephron, in the kidney, which also drives blood pressure up.

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图像

WB检测Angiotensinogen蛋白(货号 GB11535). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 500稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 5000稀释, 室温孵育1小时.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(G2025)稀释本产品.