Anti-Myeloperoxidase Rabbit pAb
￥1650
货号：YB11235-1-100
品牌：YIKE
规格：
- 100 μL
数量：
产品信息
蛋白质全称 | 髓过氧化物酶 |
别名 | MPO, myeloperoxidase |
免疫原 | 小鼠MPO重组蛋白 |
Uniprot ID | P11247 |
抗体亚型 | IgG |
纯化方式 | 亲和纯化 |
预测分子量. / 观测分子量. | 81 kDa / 60 kDa |
应用
| 应用 | 物种 | 稀释 | 阳性样品 |
| WB 免疫印迹 | 人, 小鼠, 大鼠 | 1: 500-1: 1000 | 炎症性脾 |
背景
Myeloperoxidase (MPO) is a peroxidase enzyme that in humans is encoded by the MPO gene on chromosome 17.MPO is most abundantly expressed in neutrophil granulocytes (a subtype of white blood cells), and produces hypohalous acids to carry out their antimicrobial activity. It is a lysosomal protein stored in azurophilic granules of the neutrophil and released into the extracellular space during degranulation. Neutrophil verdoperoxidase has a heme pigment, which causes its green color in secretions rich in neutrophils, such as pus and some forms of mucus.
图像
| WB检测Myeloperoxidase蛋白(货号 GB11224-1). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 500稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 5000稀释, 室温孵育1小时. |
储存
| 储存条件 | 在-20℃下储存一年, 避免反复冻融. |
| 储存缓冲液 | 含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液. |
注意:
1.本产品仅供研究使用.
2.建议用一抗稀释液(G2025)稀释本产品.
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