产品信息

蛋白质全称	线粒体NADH脱氢酶[泛醌]铁硫蛋白3
别名	Complex I-30kD, CI-30kD, NADH-ubiquinone oxidoreductase 30 kDa subunit, Ndufs3, NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, CI-30, 0610010M09Rik
Uniprot ID	Q9DCT2
免疫原	小鼠NDUFS3重组蛋白
抗体亚型	IgG
预测分子量./观测分子量.	30 kDa / 30 kDa
纯化方式	亲和纯化

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应用

应用	物种	稀释	阳性样品
WB 免疫印迹	人	1: 500-1: 1000	HeLa细胞, HepG2细胞, HEK293细胞

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背景

NDUFS3(NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial) is also named as CI-30kD and belongs to the complex I 30 kDa subunit family. It catalyzes the oxidation of NADH, with the concomitant reduction of ubiquinone and ejection of protons out of the mitochondria. NDUFS3 can be used as a mitochondrial matrix control. Mutations in this gene are associated with Leigh syndrome resulting from mitochondrial complex I deficiency.

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图像

WB检测NDUFS3蛋白(货号 GB111547). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 500稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 5000稀释, 室温孵育1小时.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(G2025)稀释本产品.