产品信息

蛋白质全称	糖基磷脂酰肌醇锚定附着1蛋白
别名	GAA1, GAA1 protein homolog, GPAA1, hGAA1, GPI anchor attachment protein 1, mGAA1, Anchor attachment protein 1 (Gaa1p, yeast) homolog, GPI transamidase subunit
Uniprot ID	Q9WTK3
免疫原	小鼠GAA1重组蛋白
抗体亚型	IgG
纯化方式	亲和纯化
亚细胞定位	内质网

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应用

应用	物种	稀释	阳性样品
IHC/IF 免疫组织化学/免疫荧光	大鼠	1: 2000-1: 4000	肾

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背景

GPAA1(Glycosylphosphatidylinositol anchor attachment 1 protein) is also named as GAA1. It is the most conspicuously hydrophobic of the known subunits of GPIT, and it may play a key role in substrate recognition. It is predicted to be a multispanning membrane protein of 67 kDa with seven transmembrane (TM) domains, a large lumenal domain between the first two TM segments, and a cytoplasmically oriented N terminus bearing a potential ER retrieval signal in the form of a diarginine motif. It has 2 isoforms produced by alternative splicing and the full length protein has 2 glycosylation sites. This antibody is specific to GPAA1.

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图像

IHC检测GAA1蛋白(货号 GB113592). 样品: 大鼠肾, 4%多聚甲醛 (货号G1101) 固定12-24小时. 抗原修复: 柠檬酸抗原修复液(干粉, pH 6.0) (G1201), 98℃, 20分钟. —抗: 1: 2000稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 200稀释, 室温孵育1小时.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(G2025)稀释本产品.