产品信息

蛋白质全称	细胞色素c氧化酶亚基6C
别名	Cytochrome c oxidase polypeptide VIc, Cox6c, EC 1.9.3.1
Uniprot ID	P09669
免疫原	KLH偶联的人COX6C合成肽
预测分子量./观测分子量.	9 kDa / 12 kDa
纯化方式	亲和纯化
偶联	辣根过氧化物酶

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应用

应用	物种	稀释	阳性样品
WB 免疫印迹	小鼠, 大鼠	1: 250-1: 1000	心
孵化条件	封闭后, 将膜与HRP标记的抗Cytochrome C Oxidase subunit VIc/COX6C 抗体 (ZB111489-HRP)在室温（20-25℃）孵育3-4小时或4℃过夜, 随后用TBST缓冲液洗涤3次, 每次5分钟.

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背景

COX6C(Cytochrome c oxidase subunit 6C) is one of the nuclear-coded polypeptide chains of cytochrome c oxidase (subunit VIc), the terminal oxidase in mitochondrial electron transport.Cytochrome c oxidase, the terminal enzyme of the mitochondrial respiratory chain, catalyzes the electron transfer from reduced cytochrome c to oxygen. It is a heteromeric complex consisting of 3 catalytic subunits encoded by mitochondrial genes and multiple structural subunits encoded by nuclear genes. The mitochondrially-encoded subunits function in electron transfer, and the nuclear-encoded subunits may be involved in the regulation and assembly of the complex.

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图像

WB检测Cytochrome C Oxidase subunit VIc/COX6C蛋白(货号 ZB111489-HRP). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. 抗体: HRP标记的抗Cytochrome C Oxidase subunit VIc/COX6C蛋白 抗体 1: 1000稀释, 4℃ 孵育过夜.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.产品颜色为浅黄色.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(G2025)稀释本产品.