产品信息

蛋白质全称	β-1,4-半乳糖基转移酶5
别名	Beta4Gal-T5, b4Gal-T5, Lactosylceramide synthase, LacCer synthase, B4galt5, Bgt-5
Uniprot ID	Q9JMK0
免疫原	KLH偶联的小鼠B4GALT5合成肽
抗体亚型	IgG
预测分子量./观测分子量.	45 kDa / 45 kDa
纯化方式	亲和纯化

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应用

应用	物种	稀释	阳性样品
WB 免疫印迹	小鼠, 大鼠	1: 1000-1: 2000	肝

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背景

Catalyzes the synthesis of lactosylceramide (LacCer) via the transfer of galactose from UDP-galactose to glucosylceramide (GlcCer) . LacCer is the starting point in the biosynthesis of all gangliosides (membrane-bound glycosphingolipids) which play pivotal roles in the CNS including neuronal maturation and axonal and myelin formation . Plays a role in the glycosylation of BMPR1A and regulation of its protein stability . Essential for extraembryonic development during early embryogenesis .

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图像

WB检测B4GALT5蛋白(货号 GB112912). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 1000稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 5000稀释, 室温孵育1小时.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(G2025)稀释本产品.