产品信息

蛋白质全称	糖基转移酶3-β-葡糖醛酸基转移酶1
别名	B3GAT1, CD57, GlcAT-P, GLCATP, GlcUAT-P, Glucuronosyltransferase P, HNK-1, HNK1, LEU7, NK 1
Uniprot ID	Q9CW73
免疫原	KLH偶联的小鼠B3GAT1合成肽
抗体亚型	IgG
纯化方式	亲和纯化
亚细胞定位	内质网, 高尔基体, 分泌型

---

应用

应用	物种	稀释	阳性样品
IHC/IF 免疫组织化学/免疫荧光	人	1: 600-1: 1200	扁桃体

---

背景

B3GAT1 plays a key role in neurodevelopment.B3GAT1 Involved in the biosynthesis of L2/HNK-1 carbohydrate epitope on glycoproteins. Can also play a role in glycosaminoglycan biosynthesis. Substrates include asialo-orosomucoid (ASOR), asialo-fetuin, and asialo-neural cell adhesion molecule. Requires sphingomyelin for activity: stearoyl-sphingomyelin was the most effective, followed by palmitoyl-sphingomyelin and lignoceroyl-sphingomyelin. Activity was demonstrated only for sphingomyelin with a saturated fatty acid and not for that with an unsaturated fatty acid, regardless of the length of the acyl group.

---

图像

IHC检测B3GAT1蛋白(货号 GB113461). 样品: 人扁桃体, 4%多聚甲醛 (货号G1101) 固定12-24小时. 抗原修复: 柠檬酸抗原修复液(干粉, pH 6.0) (G1201), 98℃, 20分钟. —抗: 1: 1000稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 200稀释, 室温孵育1小时.

---

储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

---

注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(G2025)稀释本产品.