产品信息

蛋白质全称	线粒体细胞色素c氧化酶亚基4异构体1
别名	Cytochrome c oxidase polypeptide IV, Cytochrome c oxidase subunit IV isoform 1, COX IV-1, Cox4i1, Cox4, Cox4a, COX4I1, COX4, COX4-1, COXIV, COXIV-1, COXIV-1, cytochrome c oxidase subunit 4I1
Uniprot ID	P19783
免疫原	小鼠COX IV重组蛋白
预测分子量./观测分子量.	20 kDa / 17 kDa
纯化方式	亲和纯化
偶联	辣根过氧化物酶

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应用

应用	物种	稀释	阳性样品
WB 免疫印迹	小鼠, 大鼠	1: 1000-1: 2000	脑, 肝
孵化条件	封闭后, 将膜与HRP标记的抗COX IV 抗体(ZB15250-HRP)在室温（20-25℃）孵育3-4小时或4℃过夜, 随后用TBST缓冲液洗涤3次, 每次5分钟.

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背景

COX4I1, also named as COX4 and COXIV-1, belongs to the cytochrome c oxidase IV family. It is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. COX4I1 is a marker for mitochondria. It has two isoforms (isoform 1 and 2). Isoform 1(COX4I1) is ubiquitously expressed and isoform 2 is highly expressed in lung tissues. COX4I1 is commonly used as a loading control. This antibody was generated against full length COX4I1 protein and cross reacts with COX4I2.

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图像

WB检测COX IV蛋白(货号 ZB15250-HRP). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. 抗体: HRP标记的抗COX IV蛋白 抗体 1: 2000稀释, 4℃ 孵育过夜.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.产品颜色为浅黄色.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(G2025)稀释本产品.