产品信息

蛋白质全称	腱生蛋白X
别名	Hexabrachion like protein, HXBL, Tenascin X, tenascin XB, TENX, TN X, TNX, TNXB, TNXB1, TNXB2, TNXBS, XB, XBS
Uniprot ID	O35452
免疫原	小鼠TN-X重组蛋白
抗体亚型	IgG
预测分子量./观测分子量.	435 kDa / 75 kDa
纯化方式	亲和纯化

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应用

应用	物种	稀释	阳性样品
WB 免疫印迹	小鼠, 大鼠	1: 300-1: 800	脑, 肺

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背景

Tenascin-X (TNXB) is a extracellular matrix glycoprotein predominantly located in the outer reticular lamina of the basement membrane (BM) . It interacts with many other ECM proteins and it accelerates collagen fibrillogenesisin vitro. TNXB plays a role in interactions between cell and ECM, antiadhesive effect, inhibiting cell migration, and in maintaining homeostasis of the extracellular matrix. Deficiency of TNXB has been associated with the connective tissue disorder Ehlers-Danlos syndrome. This antibody is raised against human TNXB and detects a band about 75 kDa, which probably represent a proteolytic cleaved form of human TNXB.

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图像

WB检测TN-X蛋白(货号 GB113388). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 800稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 5000稀释, 室温孵育1小时.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(G2025)稀释本产品.