产品信息

蛋白质全称	膜联蛋白A13
别名	Annexin XIII, Anxa13, Annexin, MGC150460, ANX 13, Intestine-specific, Intestine Specific Annexin, ISA,  Annexin-13
Uniprot ID	Q99JG3
免疫原	小鼠Annexin A13重组蛋白
抗体亚型	IgG
纯化方式	亲和纯化
亚细胞定位	细胞膜

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应用

应用	物种	稀释	阳性样品
IHC/IF 免疫组织化学/免疫荧光	人, 小鼠	1: 1500-1: 3000	结肠癌, 小肠

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背景

This gene encodes a member of the annexin family. Members of this calcium-dependent phospholipid-binding protein family play a role in the regulation of cellular growth and in signal transduction pathways. The Annexin A13 is considered the original progenitor of the 12 members of vertebrate Annexins. The expression of Annexin A13 is highly tissue-specific, being expressed only in intestinal and kidney epithelial cells. This expression is associated with a highly differentiated intracellular transport function.It is associated with the plasma membrane of undifferentiated, proliferating endothelial cells and differentiated villus enterocytes.

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图像

	IHC检测Annexin-13/ANXA13蛋白(货号 GB111272). 样品: 人结肠癌, 4%多聚甲醛 (货号G1101) 固定12-24小时. 抗原修复: 柠檬酸抗原修复液(干粉, pH 6.0) (G1201), 98℃, 20分钟. —抗: 1: 1500稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 200稀释, 室温孵育1小时.
	IHC检测Annexin-13/ANXA13蛋白(货号 GB111272). 样品: 小鼠小肠, 4%多聚甲醛 (货号G1101) 固定12-24小时. 抗原修复: 柠檬酸抗原修复液(干粉, pH 6.0) (G1201), 98℃, 20分钟. —抗: 1: 1500稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 200稀释, 室温孵育1小时.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(G2025)稀释本产品.