产品信息

蛋白质全称	唾液酸结合Ig样凝集素5
别名	Siglec-5, Sialic acid-binding Ig-like lectin F, Siglec-F, mSiglec-F, CD33L2, OB-binding protein 2, Sialic acid-binding Ig-like lectin F, OBBP2, CD170 antigen
Uniprot ID	Q920G3
免疫原	小鼠SIGLEC5重组蛋白
抗体亚型	IgG
预测分子量./观测分子量.	61 kDa / 70 kDa
纯化方式	亲和纯化

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应用

应用	物种	稀释	阳性样品
WB 免疫印迹	人	1: 500-1: 1000	HL6细胞0, K562细胞, H1299细胞

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背景

SIGLEC5 is a putative adhesion molecule that mediates sialic-acid dependent binding to cells. This protein binds equally to alpha2,3-linked and alpha2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. This molecule is expressed by monocytic/myeloid lineage cells. SIGLEC5 is found at high levels in peripheral blood leukocytes, spleen, bone marrow and at lower levels in lymph node, lung, appendix, placenta, pancreas and thymus.

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图像

WB检测SIGLEC5蛋白(货号 GB111590). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 500稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 5000稀释, 室温孵育1小时.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(G2025)稀释本产品.