产品信息

蛋白质全称	钙黏蛋白13
别名	Heart cadherin, Truncated cadherin, T-cad, T-cadherin, CDH13, CDHH, P105
Uniprot ID	Q9WTR5
免疫原	KLH偶联的小鼠H Cadherin合成肽
抗体亚型	IgG
预测分子量./观测分子量.	78 kDa / 78 kDa
纯化方式	亲和纯化

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应用

应用	物种	稀释	阳性样品
WB 免疫印迹	人, 小鼠, 大鼠	1: 500-1: 1000	皮质, 脊髓, 心

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背景

Cadherin13 is a member of the cadherin family of transmembrane glycoproteins that mediate calcium-dependent intercellular adhesion. However, Cadherin13 is an atypical member, lacking transmembrane and cytosolic domains and containing a GPI moiety that anchors Cadherin13 to the plasma membrane . Human Cadherin13 is synthesized as a 713 amino acid precursor that contains a 22 aa signal sequence, a 116aa propeptide, a 555aa mature chain, and a second propeptide of 20aa that is removed in the mature form to reveal the GPI anchor.

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图像

WB检测H Cadherin蛋白(货号 GB11996). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 500稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 5000稀释, 室温孵育1小时.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(G2025)稀释本产品.