产品信息

蛋白质全称	肝癌衍生生长因子
别名	HDGF, Hepatoma derived growth factor; Hepatoma-derived growth factor, High mobility group protein 1 like 2, High mobility group protein 1-like 2, HMG-1L2, HMG1L2
Uniprot ID	P51859
免疫原	小鼠HDGF重组蛋白
抗体亚型	IgG1, κ
克隆号	SB338
预测分子量./观测分子量.	37 kDa / 37 kDa
纯化方式	亲和纯化

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应用

应用	物种	稀释	阳性样品
WB 免疫印迹	人	1: 1000-1: 2000	HeLa细胞, 293T细胞, LO2细胞

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背景

This gene encodes a member of the hepatoma-derived growth factor family. The encoded protein has mitogenic and DNA-binding activity and may play a role in cellular proliferation and differentiation. This gene was thought initially to be located on chromosome X, however, that location has been determined to correspond to a related pseudogene. Alternatively spliced transcript variants encoding distinct isoforms have been described.

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图像

WB检测HDGF蛋白(货号 GB15872). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 1000稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗小鼠IgG (H+L) (货号GB23301), 1: 5000稀释, 室温孵育1小时.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(G2025)稀释本产品.