Recombinant Anti-SYT1 antibody (Rabbit mAb)
- 50 μL
产品信息
蛋白质全称 | 突触结合蛋白-1 |
别名 | Synaptotagmin I, SytI, p65, DKFZp781D2042, FLJ42519, SVP65, Syt2 |
Uniprot ID | P46096 |
免疫原 | 小鼠SYT1重组蛋白 |
抗体亚型 | IgG |
预测分子量./观测分子量. | 47 kDa / 60 kDa |
纯化方式 | 亲和纯化 |
应用
| 应用 | 物种 | 稀释 | 阳性样品 |
| WB 免疫印迹 | 人, 小鼠, 大鼠 | 1: 1000-1: 2000 | A54细胞, 脑, 大脑皮层 |
背景
Synaptotagmin 1 is a synaptic vesicle membrane glycoprotein that is widely expressed throughout the CNS and is generally thought to act as the Ca2+ sensor in the regulation of exocytosis and neurotransmitter release. Studies indicate that synaptotagmin's Ca2+ mediated binding of SNAP25 is essential for the Ca2+ dependent triggering of membrane. Synaptotagmin 1 may have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse.
图像
| WB检测SYT1蛋白(货号 GB152106). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 1000稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 5000稀释, 室温孵育1小时. |
| WB检测SYT1蛋白(货号 GB152106). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 1000稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 5000稀释, 室温孵育1小时. |
储存
| 储存条件 | 在-20℃下储存一年, 避免反复冻融. |
| 储存缓冲液 | 含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
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