Recombinant Anti-RFP antibody (Mouse mAb)
- 100 μL
产品信息
蛋白质全称 | 红色荧光蛋白drFP583 |
别名 | Red fluorescent protein, Red fluorescent protein eqFP611, RFPTag, RFP-Tag, RFP Tag |
Uniprot ID | Q9U6Y8 |
免疫原 | RFP重组蛋白 |
抗体亚型 | IgG1, κ |
克隆号 | SB168 |
纯化方式 | 亲和纯化 |
应用
| 应用 | 物种 | 稀释 | 阳性样品 |
| ICC/IF 免疫细胞化学/免疫荧光 | Species independent | 1: 500-1: 1000 | 转染的293F细胞 |
背景
Fluorescent proteins have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions.
图像
| ICC检测RFP蛋白(货号 GB155053)(红色）. 样品: 转染的293F细胞, 4%多聚甲醛 (货号G1101) 固定20分钟. 封闭: 3% BSA（货号GC305010）的PBS溶液, 室温孵育30分钟. —抗: 1: 500稀释, 4℃ 孵育过夜. 二抗: Cy3标记山羊抗兔IgG (H+L) (货号GB21303), 1: 300稀释, 室温孵育1小时. |
储存
| 储存条件 | 在-20℃下储存一年, 避免反复冻融. |
| 储存缓冲液 | 含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液. |
注意:
1.本产品仅供研究使用.
2.建议用一抗稀释液(G2025)稀释本产品.
| 货号 | 名称 | 规格 | 价格 | 操作 |
|---|
| 货号 | 名称 | 规格 | 价格 | 操作 |
|---|