产品信息

蛋白质全称	程序性细胞死亡蛋白1
别名	Protein PD-1, mPD-1, CD279, Pdcd1, Pd1, SLEB2, Systemic lupus erythematosus susceptibility 2, hSLE1, PDCD1,PD-1, PD1, SLEB2, hPD-1, hPD-l, Programmed cell death 1
Uniprot ID	Q02242
免疫原	KLH偶联的小鼠PD1合成肽
抗体亚型	IgG1, κ
克隆号	SB56
纯化方式	亲和纯化
亚细胞定位	细胞膜

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应用

应用	物种	稀释	阳性样品
IHC/IF 免疫组织化学/免疫荧光	人	1: 500-1: 1000	扁桃体

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背景

Programmed cell death 1 (PD-1, also known as CD279) is an immunoinhibitory receptor that belongs to the CD28/CTLA-4 subfamily of the Ig superfamily. It is a 288 amino acid (aa) type I transmembrane protein composed of one Ig superfamily domain, a stalk, a transmembrane domain, and an intracellular domain containing an immunoreceptor tyrosine-based inhibitory motif (ITIM) as well as an immunoreceptor tyrosine-based switch motif (ITSM) . PD-1 is expressed during thymic development and is induced in a variety of hematopoietic cells in the periphery by antigen receptor signaling and cytokines.

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图像

IHC检测PD1蛋白(货号 GB15338). 样品: 人扁桃体, 4%多聚甲醛 (货号G1101) 固定12-24小时. 抗原修复: 柠檬酸抗原修复液(干粉, pH 6.0) (G1201), 高压锅均匀喷气计时2分钟. —抗: 1: 1000稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗小鼠IgG (H+L) (货号GB23301), 1: 200稀释, 室温孵育1小时.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(G2025)稀释本产品.