产品信息

蛋白质全称	HA标签
别名	HA epitope tag, HA1, HA2, hemagglutinin, Hemagglutinin HA1 chain, Hemagglutinin HA2 chain
免疫原	KLH偶联的YPYDVPDYA合成肽
抗体亚型	IgG1, κ
克隆号	SB12
纯化方式	亲和纯化

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应用

应用	物种	稀释	阳性样品
WB 免疫印迹	Species independent	1: 1000-1: 2000	HA标签融合蛋白
ICC/IF 免疫细胞化学/免疫荧光	Species independent	1: 1000-1: 2000	转染的CHO细胞

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背景

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The HA tag is corresponds to amino acid residues YPYDVPDYA of human influenza virus hemagglutinin(HA). Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins. The HA tag is useful in western blotting and immunohistochemical localization of expressed fusion proteins when examined with antibodies raised specifically against the HA-tag.

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图像

	WB检测HA Tag(货号 GB151252). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 1000稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗小鼠IgG (H+L) (货号GB23301), 1: 5000稀释, 室温孵育1小时.
	ICC检测HA Tag(货号 GB151252)(红色). 样品: CHO细胞(转染HA标签), 4%多聚甲醛 (货号G1101) 固定20分钟. 封闭: 3% BSA（货号GC305010）的PBS溶液, 室温孵育30分钟. 一抗: 1: 2000稀释, 4℃ 孵育过夜. 二抗: Cy3标记山羊抗小鼠IgG (H+L) (货号GB21301), 1: 300稀释, 室温孵育1小时.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(G2025)稀释本产品.